Coding

Part:BBa_K1054010

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-15)

BLF1 with linker

BLF1 is the N-terminal of ampicillin resistance gene TEM-1 β-lactamase. It consists of β-lactamase amino acid 26-196. It functions together with the C-terminal of β-lactamase (BLF2, BBa_K1054011), as when they dimerize they possess ampicillinase activity. [http://www.nature.com/nbt/journal/v20/n6/full/nbt0602-619.html [1]]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None