Coding

Part:BBa_K1039030

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 integrase with Promoter and RBS

PhiC31 integrase is a serine integrase that catalyzes site-specific recombination between PhiC31 attP (BBa_K103900) and attB (Bba_K1039036) sites to produce PhiC31 attR (Bba_K1039037) and attL (Bba_K1039038) sites. This recombination is unidirectional, i.e. attR and attL do not recombine in the presence of PhiC31 integrase alone. (1). A strong promoter and RBS (BBa_K608002) has been added in front of the PhoC31 integrate. In concert with PhiC31 recombination directionality factor (RDF) (BBa_K1039013), PhiC31 integrase catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is also unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF. PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039009 and BBa_K1039008 from PB state to RL state. In concert with B PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039010 and BBa_K1039011 from RL state to PB state. References: 1. Groth, Amy C., and Michele P. Calos. "Phage Integrases: Biology and Applications." Journal of Molecular Biology. 335.3 (2004): 667-678.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1417
    Illegal SapI.rc site found at 1475
    Illegal SapI.rc site found at 1580
    Illegal SapI site found at 1726


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