Bxb1 Integrase and Recombination Directionality Factor expression cassette
A strong promoter and strong RBS (BBa_K608002) drive expression of Bxb1 integrase and Bxb1 recombination directionality factor (RDF) (BBa_K1039003 and BBa_K1039006). The RBS (BBa_B0034) is associated with RDF.
Together, Bxb1 integrase and RDF (BBa_K1039003 and BBa_K1039006) catalyze site-specific recombination between Bxb1 attR (BBa_K1039033) and attL (BBa_K1039034) sites.
They will also allow flipping of Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039004 from RL state to PB state.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 253
- 21Illegal BamHI site found at 527
Illegal XhoI site found at 614
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1166
Illegal NgoMIV site found at 1253
Illegal AgeI site found at 303
- 1000Illegal BsaI.rc site found at 1361