Plasmid_Backbone

Part:BBa_K1033200:Design

Designed by: Viktor Blomkvist   Group: iGEM13_Uppsala   (2013-08-23)


E. coli Lactobacilli shuttle vector pSBLb4C15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2958
    Illegal suffix found at 4049
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2958
    Illegal NheI site found at 1938
    Illegal SpeI site found at 4050
    Illegal PstI site found at 4064
    Illegal NotI site found at 2964
    Illegal NotI site found at 4057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2958
    Illegal BamHI site found at 1917
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2958
    Illegal suffix found at 4050
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2958
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2973
    Illegal SpeI site found at 4050
    Illegal PstI site found at 4064
    Illegal AgeI site found at 3760
    Illegal AgeI site found at 3872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Insuring it followed biobrick standard 10


Source

Ori from plasmid pJP059 inserted into pEL4C15 with ori removed

References