Part:BBa_K1023005:Experience

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Applications of BBa_K1023005

Both the codon-optimized eforRed chromoprotein encoding gene and non codon-optimized version were placed behind our pkiA promoter and in front of our xlnD terminator. This promoter and terminator allow for expression in Aspergillus niger. First we transformed Aspergillus Niger with non codon-optimized eforRed encoding gene, however no change in colour was observed. Next we designed this Aspergillus niger codon-optimized version of the eforRed chromoprotein encoding gene and transformed Aspergillus niger with it: red colour was observed. This codon-optimized eforRed encoding gene could serve as a simple selective marker or bioreporter in fungi. This is an improvement of the following part: eforRed , since we codon-optimized it for usage in Aspergillus.

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