Part:BBa_K1017726:Experience

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User Reviews

UNIQ3ffb7d744189a844-partinfo-00000000-QINU UNIQ3ffb7d744189a844-partinfo-00000001-QINU

••• 2014_WashU_StLouis

We used this part in order to activate the CcaR/CcaS light sensor system. Not 100% sure if the sequence is as depicted as we were able to get different results for our system with and without the chromophore plasmid; but when we tried to digest the part with known restriction enzymes as a crude verification of the part sequence, it would not digest properly. We also tried to amplify the backbone for biobricking purposes and would get proper piece lengths but improper sequence binding when running our sequencing PCR with primers that should have bound to the PSB1C3 backbone. Visit our project here: http://2014.igem.org/Team:WashU_StLouis

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