Reporter

Part:BBa_K079030:Design

Designed by: Giovanni Mariotta   Group: iGEM08_Bologna   (2008-10-24)


GFP reporter protein under the control of the BBa_23105 constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

The Bologna team for iGEM2008 developed the Visual Fluo software for fluorescence analysis. To test the sensitivity of the sotware we cloned the GFP reporter under the control of three promoters from the Berkley library with three different transcriptional strengths: BBa_J23105, BBa_J23118 and BBa_J23110.


Source

Registry standard parts

References