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Part:BBa_K079027:Design

Designed by: Francesca Ceroni   Group: iGEM08_Bologna   (2008-10-24)

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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2249


Design Notes

We wanted to characterize the Lac promoter repression by different levels of LacI protein. Thus, we cloned the BBa_S0100 part under the control of three promoters from the Berkley costitutive promoter library with different transcriptional strenght: BBa_J23114, BBa_J23105and BBa_J23118. As a reporter, the GFP protein (BBA_I763020) was cloned downstream of the Lac promoter (BBa_J04500). The expression level analysis after IPTG induction (100 uM) was then performed.

Source

Registry standard part

References