Part:BBa_K078005:Design
2，2‘，3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradation
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 385
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 873
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use this part to construct the degradation pathway of dioxin.
2，2‘，3-Trihydroxybiphenyl 1,2-dioxygenase is one enzyme in dioxin degradation pathway. it is one kind of Estradoil dioxygenase reponsible for meta cleavage.
This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except NOt1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37°C for about 3h.
Source
an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA
References
The reductase RedA2 of the multi-component dioxin dioxygenase system of Sphingomonas sp. RW1 is related to class-I cytochrome P450-type reductases.Jean Armengaud and Kenneth N. TIMMIS,J. Biochem. 253, 4372444 (1998)
Genetic and biochemical characterization of two new extradiol dioxygenases of Sphingomonas sp. strain RW1 and construction of two gene cassettes for biodegradation of dibenzofuran and dioxin. Von der Gemeinsamen Naturwissenschaftlichen Fakultät der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften (Dr. rer. nat.) genehmigte