Part:BBa_J72035:Design

{Bmll60} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 33
  

Design Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll60 {<V5!}

Wobble mll60/mll61                (76, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll60         {<V5!}
----
mll60    Forward wobble for {<V5!} (Bmll60)
CCATAGAATTCATGAGATCTGGTAAGCCTATCCCTAACCCTCTGCTGGGTC
mll61    Reverse wobble for {<V5!} (Bmll60)
CGTTAGGATCCTTACGTAGAATCGAGACCCAGCAGAGGGTTAG