Part:BBa_J44007:Design

pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 982
  Illegal NheI site found at 1318
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1042
  Illegal BamHI site found at 1464
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1490
  Illegal NgoMIV site found at 1858
  Illegal NgoMIV site found at 2018
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Possibility of read-thru of the pBADrev during transcription.

Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31000) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson. (BBa_J44002) - from Missouri Western State University