Plasmid

Part:BBa_I722004:Design

Designed by: Shuobo Shi   Group: iGEM07_Tianjin   (2007-08-22)


Yiplac211(-EcoRI)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 263
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 912
    Illegal SapI site found at 1064


Design Notes

The EcoRI site of this plasmid must be knocked out because the EcoRI site on it may disturbe our experiment.


Source

(pUC19)(pBR322)(yeast 2-micron plasmid)

References