Reporter

Part:BBa_G10001:Design

Designed by: Jason Kelly   Group: iGEM06_MIT   (2008-07-06)


Lux operon downstream of Plac


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6000
    Illegal XbaI site found at 2119
    Illegal XbaI site found at 6027
    Illegal PstI site found at 6039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6000
    Illegal PstI site found at 6039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6000
    Illegal BglII site found at 5440
    Illegal BamHI site found at 6021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6000
    Illegal XbaI site found at 2119
    Illegal XbaI site found at 6027
    Illegal PstI site found at 6039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6000
    Illegal XbaI site found at 2119
    Illegal XbaI site found at 6027
    Illegal PstI site found at 6039
    Illegal AgeI site found at 3097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7370
    Illegal BsaI.rc site found at 1477
    Illegal SapI site found at 3616
    Illegal SapI.rc site found at 8452


Design Notes

.


Source

pTKX15 (plasmid from tom knight)

References