IGEM:MIT/2006/Banana odor generator gas chromatograms
Verification of odor production by banana odor generator
We successfully designed, constructed and tested biosynthetic devices for banana odor production from exogenously supplied precursor. To confirm that the banana odor generator produced the correct smell compound, we analyzed cultures with the device supplemented with 5mM isoamyl alcohol by gas chromatography. The banana odor generator (BBa_J45200) produced high levels of isoamyl acetate when we added the precursor isoamyl alcohol to the culture medium (A). The cellular chassis alone (E. coli strain TOP10) did not produce isoamyl acetate although we added isoamyl alcohol to the culture medium (B). The retention time of the isoamyl acetate peak from BBa_J45200 is identical to that of the pure isoamyl acetate standard (C). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.
We successfully designed, constructed and tested biosynthetic devices for banana odor production from exogenously supplied precursor. To confirm that the banana odor generator produced the correct smell compound, we analyzed cultures with the device supplemented with 5mM isoamyl alcohol by gas chromatography. The banana odor generator (BBa_J45200) produced high levels of isoamyl acetate when we added the precursor isoamyl alcohol to the culture medium (A). The cellular chassis alone (E. coli strain TOP10) did not produce isoamyl acetate although we added isoamyl alcohol to the culture medium (B). The retention time of the isoamyl acetate peak from BBa_J45200 is identical to that of the pure isoamyl acetate standard (C). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.