IGEM:MIT/2006/Banana odor biosynthetic system gas chromatograms
No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.