Help:Assembly standard 65
Mammalian cells including human embryonic kidney (HEK-293) and chinese hamster ovary (CHO) cells are widely used in molecular and synthetic biology as model organisms for probing complex signaling pathways, designing therapeutics, interrogating the function of novel proteins, and more.
In order to harness the potential of this unique resource, MIT iGEM 2010 and 2011 have developed a library of basic promoters, transcription factors, reporters, signaling proteins, and more to provide a toolkit for the assembly of parts specifically designed for expression in mammalian cells. We call the standard used to concatenate these parts the MammoBlock standard.
Although there are currently fewer parts for mammalian cells than for bacterial or yeast cells, we at MIT expect the MammoBlock platform to transform the Registry and provide much-needed tools for cutting-edge research at the bench and beyond.
For more information about MammoBlocks, consult the official documentation found at http://dspace.mit.edu/bitstream/handle/1721.1/60082/BBFRFC65.pdf?sequence=1.