File:YbbO).png

C、IPTG(-/+) = without/with 0.2 mM IPTG for 3-6 hours, adding IPTG to a bacteria culture with OD600 0.2-0.3. M: Protein molecular weight marker ladder. Lane 2~5、7～10: pET28 plasmids encoding ybbO(BBa_K4162023)，BCMO(BBa_K4162022) without any tag were transformed into DH5α strain, single clones (ybbO1, ybbO2, BCMO1， BCMO2 ) were picked for liquid LB culture. Protein expression was induced in parallel cultures by IPTG. Bacterial cultures were monitored by OD600, and 5x10^7 cells were harvested by centrifugation and lysis in 1x SDS sample buffer. Equal amount (10 μL, 2x10^6 cells) of whole cell lysate were analyzed by SDS-PAGE (14% separation gel). Purple arrows point to ybbO protein.