File:T--goettingen--gltT complementation.jpg
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Complementation of the ΔgltT mutant strain. A. The artificial Palf4 promoter (12) and the ribosome-binding site (RBS) of the B. subtilis gapA gene were fused by PCR to the gltT gene. B. Growth of the B. subtilis wild type 168 (WT), the ΔgltT mutant BP233 and the ΔgltT Palf4-gltT complementation strain BP237 on CS-Glc minimal medium agar plates without glyphosate (- GS) and with 10 mM glyphosate (+ GS). The plates were incubated for 24 h at 37°C.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 11:27, 22 September 2018 | | 1,686 × 1,137 (213 KB) | Robertw (Talk | contribs) | Complementation of the ΔgltT mutant strain. A. The artificial Palf4 promoter (12) and the ribosome-binding site (RBS) of the B. subtilis gapA gene were fused by PCR to the gltT gene. B. Growth of the B. subtilis wild type 168 (WT), the ΔgltT mutant B... |
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