File:T--goettingen--B. subtilis Glufosinate.jpg

Glufosinate is also taken up by B. subtilis via GltT. (A) Comparison of glutamate and glufosinate. (B) Disk diffusion assay to test whether glufosinate is transported via GltT and/or GltP. The strains 168 (wild type), BP233 (ΔgltT), BP234 (ΔgltP, BP235 (ΔgltT ΔgltP), BP237 (ΔgltT amyE::Palf4-gltT) and BP236 (amyE::Palf4-gltT) were cultivated in LB medium, washed once in 1 X C-salts and propagated (100 µl cell suspension, OD600 ≈ 2) on CS-Glc minimal medium agar plates. A filter disk containing 5 µl of a 0.5 M glufosinate solution was placed in the center of the plates, which were incubated for 2 days at 37°C. (C) Evaluation of the disk diffusion assay shown in (B).