File:T--Greatbay SCIE--SDS-PAGE of FP-151.jpeg
(A) Coomassie-blue-stained SDS-PAGE analysis of Fp151 purification under through acetic acid extraction. Lanes: M, protein molecular weight marker; IS, insoluble cell debris fraction; AE, fraction extracted with 25% (v/v) acetic acid; (B). Coomassie-blue-stained SDS-PAGE analysis of Fp151 purification under denaturing conditions(). (C)Coomassie-blue-stained SDS-PAGE analysis of Fp151 His-tag affinity purification under denaturing conditions. Lanes: M, protein molecular weight marker; NC, whole-cell sample of pET28b empty vector; WC, whole-cell sample of recombinant proteins; FT, flow through after resin binding; E, eluted proteins.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 03:23, 19 October 2019 | | 1,270 × 417 (77 KB) | Jerry X (Talk | contribs) | (A) Coomassie-blue-stained SDS-PAGE analysis of Fp151 purification under through acetic acid extraction. Lanes: M, protein molecular weight marker; IS, insoluble cell debris fraction; AE, fraction extracted with 25% (v/v) acetic acid; (B). Coomassie-bl... |
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