File:Level 2 GoldenGate.jpg
Level_2_GoldenGate.jpg (554 × 338 pixels, file size: 44 KB, MIME type: image/jpeg)
Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning. The product of this assembly is multiple transcriptional units within a level 2 acceptor, creating a level two, multigene construct in which neither Bsa1 nor Bpi1 restriction sites remain.
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current | 13:53, 13 October 2014 | 554 × 338 (44 KB) | Cara.deal (Talk | contribs) | Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning. |
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