File:Experimental setup agarase assay +IPTG.png

overnight cultures of E. coli BL21 with pet15b-β-agarase and E. coli BL21 with pet15b-mcherry were grown at 37° in LB medium. To the overnight cultures as well as to the in vivo experiments, carbenicillin was added for selection and IPTG was added to induce expression. Samples were incubated for 12h at 37°C with 70 rpm shaking. E. coli BL21 with pet15b-mcherry were used as a negative control to confirm that the occurrence of reducing sugars is due to the β-agarases and not to other metabolic pathways. By using bacteria having the same plasmid but with another insert, a possible influence of the pet15b vector can also be ruled out. Created with BioRender.com