File:Encapsulins SDS.png

SDS PAGE of the different encapsulin proteins expressed by iGEM EPFL 2018. Before (B) heat purification, the pellet after heat purification (P) and the supernatant after heat purification (S). From left to right: 1-3 Negative control (cell-free PURE expression without DNA), 4-6 HexaHis Encapsulin ([[1]]) showing bands for the encapsulin multimer high on the gel lanes as well as the monomer around 31kDa, 7 HexaHis-OVA Encapsulin ([[2]]) where bands are not easily discernible, 8 Ladder, 9 HexaHis-OVA Encapsulin ([[3]]) where the monomer band is visible at 31kDa, 10-12 HexaHis Encapsulin ([[4]]) where the bands for the 60-mer and monomer can be identified, 13-15 HexaHis-OVA Encapsulin ([[5]]) where the bands can easily be discerned for both the monomer and 60-mer (note how the 60-mer band is more visible in the supernatant after heat purification, this suggests that the crowded cell free expression environment is not as conducive to encapsulin self assembly)