File:Cre+loxP.png
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Figure 2. The verification of DNA cleavage between LoxP sites. Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 16:55, 21 October 2019 | | 373 × 217 (40 KB) | YY12138 (Talk | contribs) | Reverted to version as of 16:51, 21 October 2019 |
| 16:52, 21 October 2019 |  | 373 × 217 (40 KB) | YY12138 (Talk | contribs) | ||
| 16:51, 21 October 2019 |  | 373 × 217 (40 KB) | YY12138 (Talk | contribs) | ||
| 04:18, 18 October 2019 |  | 373 × 217 (40 KB) | YY12138 (Talk | contribs) | Figure 2. The verification of DNA cleavage between LoxP sites. Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product... |
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