File:Conformation.jpg
BBa_K1424000 & BBa_K1424001 were used (flanking a kanamycin resistance cassette designed for Synechocystis sp. PCC 6803) as double homologous recombination sequences for insertion of exogenous DNA into the genome of Synechocystis sp. PCC 6803. Colonies were picked and segregated on kanamycin containing BG11 growth media. The inclusion of exogenous DNA into the host genome was verified by performing a band-shift colony PCR. Using left homologous region forward primer and right homologous region reverse primer, PCR amplification of this genomic region can be performed. The increase in band size (band shift) of the mutant strain respective of the wild type strain confirms integration of exogenous kanamycin resistance cassette into the host genome.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 10:04, 14 October 2014 | | 776 × 302 (40 KB) | Lamja264 (Talk | contribs) | BBa_K1424000 & BBa_K1424001 were used (flanking a kanamycin resistance cassette designed for Synechocystis sp. PCC 6803; BBa_K1424003) as double homologous recombination sequences for insertion of exogenous DNA into the genome of Synechocystis sp. PCC 680 |
| 09:44, 14 October 2014 |  | 776 × 302 (40 KB) | Lamja264 (Talk | contribs) | BBa_K1424000 & BBa_K1424001 were used (flanking a kanamycin resistance cassette designed for Synechocystis sp. PCC 6803) as double homologous recombination sequences for insertion of exogenous DNA into the genome of Synechocystis sp. PCC 6803. Colonies w |
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