File:BBa K649402 graph(2).png

Urea concentration in growth media 1 hour after IPTG induction.

Introduction of BBa_K649402 containing arginase coding gene (rocF) and Arg Box (an arg operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (argR +) and strain JD24293 (argR -). But in both strains, the amount urea produced was not much different from that when BBa_K649301 containing rocF but not Arg box) was introduced. This result shows that rocF is working but Arg box is not working efficiently in this part. The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.
This work is done by Natsuki Kubo.