File:BBa K1897015 LuxR+I Composite and BBa K1897017 Inv+GFP Composite.png
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In order to check that the 3A ligation was successful for luxR+luxI as well as invasin+GFP, a restriction enzyme (RE) digestion was performed using EcoRI and SpeI on the individual plasmids. The boxed up fragments in the gel photo indicates the correct band sizes, while a control was done to ensure that the digestion was successful. The expected band sizes for luxR+luxI was around 1900 bp, while the expected band size for invasin+GFP was around 4200 bp.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 18:17, 15 October 2016 | | 807 × 695 (522 KB) | Shihuiangle (Talk | contribs) | In order to check that the 3A ligation was successful for luxR+luxI as well as invasin+GFP, a restriction enzyme (RE) digestion was performed using EcoRI and SpeI on the individual plasmids. The boxed up fragments in the gel photo indicates the correct... |
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