File:BBa K1897011 Invasin Composite WB.png
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Induction of invasin with pLuxR. The bacteria was co-transformed with BBa_K1897017 and BBa_K1897008, and and invasin protein induction was done by adding 100 μM of N-(3-oxo-hexanoyl)-homoserine lactone (AHL) to the transformed bacteria and incubated overnight (at 37 degrees with shaking). The cells were then lysed and centrifuged. Western blot for invasin was done on both the lysate and pellet obtained, and a control was also done without addition of AHL and also lysed and centrifuged. The expected protein band is boxed up (expected size: 108 kDa), and two clones (#2 and #17) were used.
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| current | 20:13, 20 October 2016 | | 1,097 × 846 (174 KB) | Shihuiangle (Talk | contribs) | Induction of invasin with pLuxR. The bacteria was co-transformed with <partinfo>BBa_K1897017</partinfo> and <partinfo>BBa_K1897008</partinfo>, and and invasin protein induction was done by adding 100 μM of N-(3-oxo-hexanoyl)-homoserine lactone (AHL) t... |
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