File:BBa Characterization LiU.pdf

Characterization of BBa_750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate reader at 37*C. Between measurements the plate was shaking with 216 rpm. Cells with BBa_750000 were done in 12 replicates (4 technical and 3 biological (different clones from the same agarplate), the empty cells received the same amounts of arabinose as the cells with BBa_750000 but were measured in technical duplicate (2 of each conc.) and the eGFP reference all were induced with 0.2 mM IPTG (12 technical replicates).