File:4 MicroscopyImages-updated.png

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MATLAB Pseudocode for each frame across timelapse:

   Apply Gaussian filter to reduce noise.
   Remove image artifacts by Otsu’s threshold method.
   Calculate location of cell center and use average radius
   Analyze fluorescence by normalized whole frame & single cell

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current06:20, 9 December 2015Thumbnail for version as of 06:20, 9 December 20151,434 × 1,059 (1.23 MB)Mbecich (Talk | contribs)MATLAB Pseudocode for each frame across timelapse: Apply Gaussian filter to reduce noise. Remove image artifacts by Otsu’s threshold method. Calculate location of cell center and use average radius Analyze fluorescence by normalized whol
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