DNA/Chromosomal integration
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The AmyE integration DNA parts (BBa_K143001 and BBa_K143002) are two parts that can be added to the 5' and 3' ends of a construct to allow integration into the B. subtilis genome. These parts have been successfully used within the parts BBa_K143079 and BBa_K143082 for integration. Integrated synthetic biological systems offer better genetic stability and more regulated copy number than plasmid-borne systems. For more information about these parts, please see [http://2008.igem.org/Team:Imperial_College 2008 Imperial College iGEM team wiki.] |
| Name | Description | Locus | Length |
|---|---|---|---|
| BBa_K143001 | 5 Integration Sequence for the amyE locus of B. subtilis | 522 | |
| BBa_K143002 | 3 Integration Sequence for the amyE locus of B. subtilis | 1002 |