Cell-free chassis/Vesicle

< Back to Cell-free chassis


Cell-Free
Systems (back to Intro) 		Introduction
Chassis
description
Vesicle Specifications.JPG
Preparation
protocol
Vesicle Encapsulation.JPG
Pictorial representation of method for vesicle-encapsulation. This method is based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005).
Preliminary
Testing
Vesicle With Expression.JPG
Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial E. coli S30 extract together with the DNA constuct BBa_T9002. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).

References

<biblio>

  1. 1 pmid=12963816
  2. 2 pmid=16224117

</biblio>