Chassis/B.subtilis Strains/B.subtilis chassis


B.subtilis strains B. subtilis chassis characterisation B. subtilis specific parts Key Parts and Devices

Bacillus subtilis chassis characterisation

The characterisation of a chassis allows system and device designers to better understand how a chassis beahves and responds, making it possible to design systems that are better tailored to the host. All current characterisation is of the B. subtilis strain 168 but should be roughly similar to that of most B. subtilis laboratory strains.

As more labs and groups use B. subtilis the characterisation and understanding of different subspecies of B. subtilis will increase. As there is an increase in characterisation of different strains and in particular their differences, the best strain for a given task can be selected.

Strain 168

Growth

The Imperial College 2008 team developed a simple model for the growth of B. subtilis where the rate of growth is related to the amount of nutrients available. More details about this model and the results can be found by [http://2008.igem.org/Team:Imperial_College/Growth_Curve clicking this link]
Nutrient ft.JPG
Label Model.JPG



Hill fn eg.JPG
GROWTH CONSTANT (A): 1.3494 HILL COEFFICIENT (n): 1.25




Motility

The Imperial College iGEM 2008 team carried out a detailed analysis of the swimming motility of B. subtilis, which led us, among other things, to develop a simple mechanical model for the swimming motility of B. subtilis. Using manual tracking, x,y coordinate data was extracted from the cell trajectory. This has allowed fitting of experimental data with the model and suggests that flagellar force of B. subtilis is Exponentially distributed. For more details about the model and the tracking software please [http://2008.igem.org/Team:Imperial_College/Motility click this link]