Antibiotic Resistance

Antibiotic Resistance of the pSB1Ax3 Plasmids

	Tet	Kan	Cm
pSB1AT3	150ug/ml	5 ug/ml	3.5 ug/ml
pSB1AK3	1.5 ug/ml	5000 ug/ml	3.5 ug/ml
pSB1AC3	1.5 ug/ml	5 ug/ml	3500 ug/ml
Normal Plates (1 L volume)
Antibiotic	3ml	1 ml	1 ml
Antibiotic	@5 mg/ml	@ 50 mg/ml	35 mg/ul
Normal	15 ug/ml	50 ug/ml	35 ug/ml

The normal plate recipe is the specified volume of antibiotic at the specified antibiotic strength in one Liter of agar broth. This results in the specified concentration of antibiotic in the plates.

In all cases, the antibiotic concentration is selected to be 10 times the amount required to select against cells that have been transformed with the wrong plasmid.

In all cases, cells transformed with the correct plasmid are able to tolerate at least 10 times this concentration of antibiotic.

Antibiotic resistances were measured by Tom Knight and Steve Payne (6/6/2005).