Plasmid_Backbone
pSB1K3

Part:pSB1K3

Designed by: Austin Che   Group: iGEM07_Example   (2008-09-08)


High copy BioBrick assembly plasmid

This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2183
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2189
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2183
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2183
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2183
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2198
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2183
    Illegal XbaI site found at 2198
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
originpMB1
resistanceK