Regulatory
OmpR

Part:BBa_R0083

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-27)


Promoter (OmpR, positive)

Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.


Usage and Biology

This promoter contains upstream regions of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC.

Activation of ompC has been experimentally shown to occur with just the C1 OmpR binding region, provided that the C1 region is placed next to the -35 signal (Maeda and Mizuno, 1990). This promoter is a truncated version of BBa_R0082, which contains C1, C2, and C3.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/uncategorized
Parameters
biology
directionForward
negative_regulators
positive_regulators1