Difference between revisions of "Part:BBa K864402:Experience"

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2019 iGEM team Linkoping Sweden validated this part.<br><br>
 
2019 iGEM team Linkoping Sweden validated this part.<br><br>
  
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[[Image:T--Linkoping_Sweden--pcons-eforred.jpeg|460px]]
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[[Image:T--Linkoping_Sweden--eforrudtsgfytdpl.jpeg|460px]]
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<i><b>Figure 1.</b></i> <b>To the left</b> This biobrick after 48 hours in 37 degrees Celsius (both pictures). To the left is cultured E. coli BL21 cells with this biobrick in white light. The culture tubes had a constant supply of oxygen (cotton plugs) which is important for the chromophore/fluorophore of eforRed to develop. <b>To the right</b> is colonies in the same host as before illuminated in 302 nm UV-light, showing eforReds ability to also emit strong fluorescence.
  
[[File:T--Linkoping_Sweden--eforrudtsgfytdpl.jpeg|300px|thumb|left|<b>Figure 1.</b> This biobrick after 48 hours in 37 degrees Celsius. Bacterial host in the picture is E. coli BL21 DE3 Gold. The picture is with the plate in 302 nm UV light, which also shows eforReds fluorescence.]]
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[[File:T--Linkoping_Sweden--eforredvsmngphoto.jpeg|300px|thumb|center|<b>Figure 1.</b> The biobrick in a 96-well plate (left), the culture has been in the well O.N in 37 degrees Celsius with a constant access to oxygen. The plate to the right is different expression systems used to test a green fluorescent protein. These results show eforReds ability to fluorescence, making it a fluorescent as well as a chromoprotein.]]
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[[Image:T--Linkoping_Sweden--eforredoxygen.png|460px]]
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[[Image:T--Linkoping_Sweden--eforredvsmngphoto.jpeg|460px]]
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<i><b>Figure 2.</b></i> <b>To the left</b> is a spectrophotometric experiment where varying levels of oxygen supply were tested. The holes were made in the plastic cover of a 96-well plate and run for 16 hours in 37 degrees Celsius, this results shows the oxygen dependency of eforReds chromophore/fluorophore development. <b>To the right</b> is this biobrick in a 96-well plate, the culture has been in the well O.N in 37 degrees Celsius with varying levels of oxygen supply. The plate to the right is different expression systems used to test a strong green/yellow fluorescent protein. These results show eforReds ability to fluorescence, making it a fluorescent as well as a chromoprotein. Both plates were illuminated in 302 nm UV-light.
  
[[File:T--Linkoping Sweden--pcons-eforred.jpeg|300px|thumb|right|<b>Figure 1.</b> This biobrick after 48 hours in 37 degrees Celsius. Bacterial host in the picture is E. coli BL21 DE3 Gold. This biobrick is to the left and a control (wild-type BL21) is to the right. The cultured tubes had a constant access to oxygen.]]
 
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===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K864402 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K864402 StartReviews</partinfo>

Revision as of 03:42, 15 August 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K864402

2019 iGEM team Linkoping Sweden

2019 iGEM team Linkoping Sweden validated this part.

T--Linkoping Sweden--pcons-eforred.jpeg T--Linkoping Sweden--eforrudtsgfytdpl.jpeg

Figure 1. To the left This biobrick after 48 hours in 37 degrees Celsius (both pictures). To the left is cultured E. coli BL21 cells with this biobrick in white light. The culture tubes had a constant supply of oxygen (cotton plugs) which is important for the chromophore/fluorophore of eforRed to develop. To the right is colonies in the same host as before illuminated in 302 nm UV-light, showing eforReds ability to also emit strong fluorescence.



T--Linkoping Sweden--eforredoxygen.png T--Linkoping Sweden--eforredvsmngphoto.jpeg

Figure 2. To the left is a spectrophotometric experiment where varying levels of oxygen supply were tested. The holes were made in the plastic cover of a 96-well plate and run for 16 hours in 37 degrees Celsius, this results shows the oxygen dependency of eforReds chromophore/fluorophore development. To the right is this biobrick in a 96-well plate, the culture has been in the well O.N in 37 degrees Celsius with varying levels of oxygen supply. The plate to the right is different expression systems used to test a strong green/yellow fluorescent protein. These results show eforReds ability to fluorescence, making it a fluorescent as well as a chromoprotein. Both plates were illuminated in 302 nm UV-light.







User Reviews

UNIQdc970c77a96a0da7-partinfo-00000000-QINU UNIQdc970c77a96a0da7-partinfo-00000001-QINU