Coding
GFP_His

Part:BBa_K863121

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the untagged version for source information.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
colorGreen
emission511
excitation501
lum
proteinGFPmut3b
tagHis6