Coding
GFP_Freibu

Part:BBa_K863120

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the original version for source information.

Usage and Biology

GFP (mut3b)

BioBrick has not yet been used. Built to be fused to binding domains for characterization. Standard 25 restriction-sites are at both ends to switch order of GFP and fused protein.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 650


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
colorGreen
emission511
excitation501
lum
proteinGFPmut3b
tagNone