Composite

Part:BBa_K824012

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-02)


Lead Promoter with GFP Reporter (Lead Detector)

We digested the promoter responsive to Lead (BBa_I721001) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter and reporter were Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production following the addition of Lead Nitrate.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 744


[edit]
Categories
//function/sensor/metal
Parameters
None