Composite

Part:BBa_K824008

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-01)

Cadmium Promoter with GFP Reporter

We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production following the addition of Cadmium.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 855


[edit]
Categories
//function/sensor/metal
Parameters
None