Plasmid_Backbone
pSBBs2E

Part:BBa_K823027

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-21)

pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.

This version contains neither NgoMIV, nor AgeI restriction sites, therefore is RFC25-compatible.


This backbone is a BioBricked version of the B. subtilis vector pAX01. It was also evaluated in the publication The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis by Radeck et al..

This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox

Reference: Härtl et al.



Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 91
    Illegal NotI site found at 1622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 2591
    Illegal NgoMIV site found at 4919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4743


[edit]
Categories
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/operation
Parameters
chassisB. subtilis + E. coli
copies
origin
resistanceA(E. coli) E(B. subtilis)