Plasmid_Backbone
pSBBs4S

Part:BBa_K823024

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-21)

pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis


This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This backbone is a BioBricked version of the B. subtilis vector pXT.

LMU-Munich-PSBBs4S-Pxyl.png

CAUTION: The submitted BioBrick can somehow not be transformed into Bacillus subtilis. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!

This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox

Reference: Derré et al.



Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4773
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4773
    Illegal BamHI site found at 4757
    Illegal XhoI site found at 1011
    Illegal XhoI site found at 1929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4773
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4773
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4788
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1893
    Illegal NgoMIV site found at 3032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2856
    Illegal EcoRI site found at 4773
    Illegal XbaI site found at 4788
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmid/expression
//plasmidbackbone/expression/inducible
Parameters
chassisB. subtilis + E. coli
copies
origin
resistanceA(E. coli) S(B. subtilis)