Coding

Part:BBa_K823020

Designed by: Simon Kelterborn   Group: iGEM12_LMU-Munich   (2012-08-15)

chloramphenicol acetyltransferase (cat)

This part codes for the chloramphenicol acetyltransferase (cat) used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions.

NOTE: This part is delivered in pSB1A3. See part design

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//cds/selectionmarker/antibioticresistance
//chassis/prokaryote/bsubtilis
Parameters
biologyBacillus subtilis
proteincat
resistanceC