This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
Usage and Biology
It is an amphipathic protein with 308 long amino acid residues, having 22 residues long hydrophobic region near N-terminus. Reports have shown that membranes have limited capacity to hold this protein, and insertion of MotB in membrane requires other components, including MotA protein.
Stader, J., Matsumura, P., Vacante, D., Dean, G. and Macnab, R., 1986. Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. Journal of Bacteriology, 166(1), pp.244-252.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 310
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.