GFP with Standard 25 Prefix/Suffix
This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
UCL iGEM 2017 used this BioBrick for its designs and noticed that this BioBrick actually contains the GFP protein reporter (E0040) fused to a FLAG epitope tag, followed by an enterokinase cleavage site. This is important if cell surface expression of this BioBrick is wished. Addition of enterokinase (also called enteropeptidase) to the solution allows easy testing of protein localisation (inside or outside of the membrane).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 641