Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
lux pl-RBS-C0062-B0015 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreasing the transcriptional rate of the lux pL promoter.
The quorum sensing molecule AHL is produced by LuxI which is under the control of the lux pR promoter. In combination with LuxR, AHL thus increases the production of AHL, a positive feedback loop.
The GFP expression rapidly increases, indicating functionality of this part. The formed AHL from LuxI forms a complex with LuxR and induces pR, that controls GFP expression. As a result, a positive feedback loop is initiated.
There's a small note regarding the biosafety of this BioBrick composite part. Upon release into the environment (this includes infection of a laboratory worker) this BioBrick composite part might alter the pathogenicity of pathogens that rely on quorum sensing to infect hosts. Pathogenic species that might be involved can be found on the following external biological safety page.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1821
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1101
|control||(1) R0063 & (2) R0062|
|protein||(1) LuxR & (2) luxI-LVA|