The gene encoding fructose-1,6-bisphosphatase class I
Usage and Biology
This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase (FBPase) to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate.
This coding region has significant alignment with other categorized Synechococcus strains, as shown in the nucleotide blast result of the provided sequence through https://blast.ncbi.nlm.nih.gov/Blast.cgi (Figure 1).
Although this sequence possesses several nucleotide sequences that make it BioBrick incompatible, it is a vital central metabolism gene that future teams may want to work with. Relevant silent mutations could resolve this incompatibility and be a great contribution for future teams.
Sequence and Features
- 10Illegal PstI site found at 493
- 12Illegal PstI site found at 493
Illegal NotI site found at 746
- 21Illegal BglII site found at 36
Illegal BglII site found at 656
- 23Illegal PstI site found at 493
- 25Illegal PstI site found at 493
- 1000Illegal BsaI.rc site found at 22