Project

Part:BBa_K404100

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)

[AAV2]-left-ITR

[AAV2-left-ITR]
BioBrick Nr. BBa_K404100
RFC standard RFC 10
Requirement pSB1C3
Source pAAV_MCS provided by Stratagene
Submitted by FreiGEM 2010
















This BioBrick is a very GC-rich sequence with several palindromic motifs forming strong hairpin structures. By using the Virus Construction Kit of the Freiburg iGEM team 2010, any gene of interest, which is cloned between this part and the right ITR can be packaged into the AAV2 capsid. These recombinant AAV2 particles can be used for different applications, and were mainly created for therapeutic usage, including cancer treatment with prodrug activating enzymes or imaging approaches.


Usage and Biology

The AAV2 ITRs serve as primers for the host cells’ DNA polymerase which converts the single-stranded virus genome into double-stranded DNA as a part of the viruses replicative cycle. They also play important roles in the virus integration into and rescue from the hosts genome, the formation of concatamers in the host cell nucleus and the encapsidation of the viral genome(K. Berns 1990). Due to these essential functions, the ITR structures cannot be deleted from a viral vector and need to be delivered in cis.


Freiburg10 VectorplasmidBrick 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//function/regulation/transcriptional
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/vector_plasmid
Parameters
None