Plasmid_Backbone

Part:BBa_K3935000

Designed by: Stefanos Karagiannakis   Group: iGEM21_Crete   (2021-09-28)


pBluescript II SK(-)_BsaI domesticated

Standard cloning vector (phagemid excised vector from lambda ZAPII). The f1(-) orientation allows rescue of antisense strand ssDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 702
    Illegal XbaI site found at 732
    Illegal SpeI site found at 726
    Illegal PstI site found at 708
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 702
    Illegal SpeI site found at 726
    Illegal PstI site found at 708
    Illegal NotI site found at 738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 702
    Illegal BamHI site found at 720
    Illegal XhoI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 702
    Illegal XbaI site found at 732
    Illegal SpeI site found at 726
    Illegal PstI site found at 708
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 702
    Illegal XbaI site found at 732
    Illegal SpeI site found at 726
    Illegal PstI site found at 708
    Illegal NgoMIV site found at 129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1031


[edit]
Categories
Parameters
None